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abi 392 dna rna synthesizer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher abi 392 dna rna synthesizer
    Abi 392 Dna Rna Synthesizer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/abi+392+dna+synthesizer/DNA/pmc11388086__SC___015___D4SC05175K___s001-248-6-10
    Average 99 stars, based on 1 article reviews
    abi 392 dna rna synthesizer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    DNA Synthesis:

    Article Title: Structural and Biochemical Analysis of DNA Helix Invasion by the Bacterial 8-Oxoguanine DNA Glycosylase MutM
    Article Snippet: .. DNA Synthesis and Purification—All of the DNA substrates were synthesized using solid phase synthesis on an ABI 392 DNA synthesizer using standard reagents and protocols (Applied Biosystems and Glen Research). .. For the cross-linkermodified oligonucleotides, the protocol was modified to include a 3 H-phosphonate (Glen Research) in the coupling step at the site of the backbone modification.

    Article Title: Structural and Biochemical Analysis of DNA Helix Invasion by the Bacterial 8-Oxoguanine DNA Glycosylase MutM
    Article Snippet: .. DNA Synthesis and Purification All of the DNA substrates were synthesized using solid phase synthesis on an ABI 392 DNA synthesizer using standard reagents and protocols (Applied Biosystems and Glen Research). .. For the cross-linker-modified oligonucleotides, the protocol was modified to include a 3′ H-phosphonate (Glen Research) in the coupling step at the site of the backbone modification.

    Purification:

    Article Title: Structural and Biochemical Analysis of DNA Helix Invasion by the Bacterial 8-Oxoguanine DNA Glycosylase MutM
    Article Snippet: .. DNA Synthesis and Purification—All of the DNA substrates were synthesized using solid phase synthesis on an ABI 392 DNA synthesizer using standard reagents and protocols (Applied Biosystems and Glen Research). .. For the cross-linkermodified oligonucleotides, the protocol was modified to include a 3 H-phosphonate (Glen Research) in the coupling step at the site of the backbone modification.

    Article Title: Structural and Biochemical Analysis of DNA Helix Invasion by the Bacterial 8-Oxoguanine DNA Glycosylase MutM
    Article Snippet: .. DNA Synthesis and Purification All of the DNA substrates were synthesized using solid phase synthesis on an ABI 392 DNA synthesizer using standard reagents and protocols (Applied Biosystems and Glen Research). .. For the cross-linker-modified oligonucleotides, the protocol was modified to include a 3′ H-phosphonate (Glen Research) in the coupling step at the site of the backbone modification.

    Synthesized:

    Article Title: Structural and Biochemical Analysis of DNA Helix Invasion by the Bacterial 8-Oxoguanine DNA Glycosylase MutM
    Article Snippet: .. DNA Synthesis and Purification—All of the DNA substrates were synthesized using solid phase synthesis on an ABI 392 DNA synthesizer using standard reagents and protocols (Applied Biosystems and Glen Research). .. For the cross-linkermodified oligonucleotides, the protocol was modified to include a 3 H-phosphonate (Glen Research) in the coupling step at the site of the backbone modification.

    Article Title: Structural and Biochemical Analysis of DNA Helix Invasion by the Bacterial 8-Oxoguanine DNA Glycosylase MutM
    Article Snippet: .. DNA Synthesis and Purification All of the DNA substrates were synthesized using solid phase synthesis on an ABI 392 DNA synthesizer using standard reagents and protocols (Applied Biosystems and Glen Research). .. For the cross-linker-modified oligonucleotides, the protocol was modified to include a 3′ H-phosphonate (Glen Research) in the coupling step at the site of the backbone modification.

    Article Title: Intertwining DNA-RNA nanocapsules loaded with tumor neoantigens as synergistic nanovaccines for cancer immunotherapy
    Article Snippet: .. DNA was synthesized using reagents from Glen Research (Sterling, VA) and Chemgenes (Wilmington, MA), on an Applied Biosystems ABI 392 DNA synthesizer. .. DNA was deprotected according to manufacturer’s instructions, followed by purification on a RP-HPLC (Dionex Ultimate 3000, ThermoFisher Scientific, Waltham, MA).

    Article Title: Structural and Biochemical Analysis of DNA Helix Invasion by the Bacterial 8-Oxoguanine DNA Glycosylase MutM
    Article Snippet: Abasic site substrates were prepared by pretreating a 5′- 32 P-labeled uracil-containing 16-mer duplex DNA (5′-AGCGTCCAUGTCTACC-3′) with uracil DNA glycosylase (New England Biolabs) at 37 °C for 1 h. The abasic site cleavage reaction was carried out using 100 n m pretreated DNA duplex and 100 n m wild-type, F114A, or M77A MutM in a reaction buffer of 40 m m NaCl and 50 m m Tris-HCl, pH 7.4, at room temperature. .. All of the DNA substrates were synthesized using solid phase synthesis on an ABI 392 DNA synthesizer using standard reagents and protocols (Applied Biosystems and Glen Research). .. Point mutations of Bst MutM were made using the QuikChange site-directed mutagenesis kit (Stratagene), and the mutations were confirmed by sequencing.

    Article Title: Albumin/vaccine nanocomplexes that assemble in vivo for combination cancer immunotherapy
    Article Snippet: .. DNA was synthesized at a 1 μmole scale of solid phase synthesis on an ABI 392 DNA synthesizer (Applied Biosystems) using materials purchased from Glen Research (Sterling, VA) or Chemgenes (Wilmington, MA). .. DNA was deprotected accordingly to the manufacturer’s instructions, and purified by reverse phase HPLC (Dionex Ultimate 3000, Thermo Fisher Scientific, Waltham, MA).

    Article Title: Albumin/vaccine nanocomplexes that assemble in vivo for combination cancer immunotherapy
    Article Snippet: .. DNA was synthesized at a 1 μmole scale of solid phase synthesis on an ABI 392 DNA synthesizer (Applied Biosystems) using materials purchased from Glen Research (Sterling, VA) or Chemgenes (Wilmington, MA). .. DNA was deprotected accordingly to the manufacturer’s instructions, and purified by reverse phase HPLC (Dionex Ultimate 3000, Thermo Fisher Scientific, Waltham, MA).

    other:

    Article Title: Structural and Biochemical Analysis of DNA Helix Invasion by the Bacterial 8-Oxoguanine DNA Glycosylase MutM
    Article Snippet: Abasic site substrates were prepared by pretreating a 5′- 32 P-labeled uracil-containing 16-mer duplex DNA (5′-AGCGTCCAUGTCTACC-3′) with uracil DNA glycosylase (New England Biolabs) at 37 °C for 1 h. The abasic site cleavage reaction was carried out using 100 n m pretreated DNA duplex and 100 n m wild-type, F114A, or M77A MutM in a reaction buffer of 40 m m NaCl and 50 m m Tris-HCl, pH 7.4, at room temperature.

    Article Title: Structural and Biochemical Analysis of DNA Helix Invasion by the Bacterial 8-Oxoguanine DNA Glycosylase MutM
    Article Snippet: Point mutations of Bst MutM were made using the QuikChange site-directed mutagenesis kit (Stratagene), and the mutations were confirmed by sequencing.



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